七星彩开奖结果

由于环境因素和细胞内的正常代谢过程造成的DNA损伤,每个细胞每天都会发生1,000到1,000,000个。虽然这些只占人类基因组约60亿个碱基中的一小部分,但如果关键基因损伤未及时修复可能会阻碍细胞的正常生理功能,进而增加癌变可能。彗星实验,或称单细胞凝胶电泳(Single cell gel electrophoresis,SCGE,是一种测量单个细胞DNA损伤的常用技术。其原理简单,即在电泳场,将受损细胞DNA(包含片段和链断裂)与完整的DNA分离通过显微镜观察到损伤细胞呈现出典型的彗星状尾巴,然后通过测量计算彗尾大小对比出细胞DNA损伤的程度因为彗星实验特点,该方法几乎被用来评估任何类型的真核细胞的 DNA 修复能力,包括双、单链断裂的不同的 DNA 损伤情况。是一种能快速、大通量检测真核细胞DNA损伤进而判别遗传毒性的技术。

彗星实验结果图

彗星实验原理简单,但操作繁琐,需要丰富的实验经验和技巧,尤其常常出现的“脱胶”问题,困扰了科研人员。除此之外,有时为了跑出完美的“彗星”图案放在paper里,还需要重复做许多次实验,费时费力。为了解决上述问题,我们推荐CellBiolabsOxiSelectTMComet Assay Kit即彗星实验试剂盒来检测细胞的DNA损伤。该试剂盒不仅能让彗星实验化繁为简,还两种不同规格(3孔和96孔)的细胞电泳凝胶板供选择让少量样本和大量样本的DNA损伤检测通通轻松hold住。用该试剂做彗星实验流程如下图。

除了操作简便,OxiSelectTMComet Assay Kit还有以下优点:

1)      适用于各种DNA损伤检测,是一款非常好用的DNA损伤检测筛选工具;

2)      试剂盒中的载玻片经过特殊处理以粘附低熔点琼脂糖,避免“脱胶”问题出现

3)      采用特殊的DNA荧光染料,能有效降低背景干扰,更加方便读取实验结果。


彗星实验试剂盒信息:

品名

货号

规格

说明

OxiSelectTM Comet Assay Kit (3-Well Slides)

STA-350

15 assays

试剂盒内有53孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测15个样品。

OxiSelectTM Comet Assay Kit (3-Well Slides)

STA-351

75 assays

试剂盒内有25张3孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测75个样品。

OxiSelectTM Comet Assay Kit (96-Well Slides)

STA-355

96 assays

试剂盒内有1张96孔载玻片和彗星实验所需的低熔点琼脂糖、裂解液及DNA荧光染料,共可检测96个样品。



为了
满足客户更多样的实验需求,彗星实验试剂盒特殊处理电泳载玻片可以单独购买,详情如下:

 


品名

货号

规格

产品图片

Comet Assay Slides, 3-Well

STA-352

5 slides

STA-353

25 slides

Comet Assay Slides, 96-Well

STA-356

1 slides

STA-356-5

5 slides

产品部分发表文献:

Maiuri, T. et al. (2016). Huntingtin is a scaffolding protein in the ATM oxidative DNA damage response complex. Hum. Mol. Genet. doi:10.1093/hmg/ddw395.

Irianto, J. et al. (2016). Nuclear constriction segregates mobile nuclear proteins away from chromatin. Mol. Bio. Cell doi:10.1091/mbc.E16-06-0428.

Andronescu, E. et al. (2016). Nanomaterials for medical applications: Benefits and risks. J Nanomater. doi:10.1155/2016/8284319.

Liu, Z。 et al。 (2016)。 Canonical microRNAs enable differentiation, protect against DNA damage, and promote cholesterol biosynthesis in neural stem cells。 Stem Cells and Dev。 doi:10。1089/scd。2016。0259。

Dai, C. et al. (2016). Curcumin ameliorates furazolidone-induced DNA damage and apoptosis in human hepatocyte L02 cells by inhibiting ROS production and mitochondrial pathway. Molecules. 21:1061.

Beegle, J. R. et al. (2016). Preclinical evaluation of mesenchymal stem cells overexpressing VEGF to treat critical limb ischemia. Mol Ther Methods Clin Dev.doi:10.1038/mtm.2016.53.

Suzuki, Y。 et al。 (2016)。 Pharmacodynamics of anti-inflammatory drugs–intranasal corticosteroid and dna damage。 Pharmacodynamics of anti-inflammatory drugs。 117-126。

Zhang, J. et al. (2016). Inhibition of Glucose-6-Phosphate Dehydrogenase could Enhance 1, 4-Benzoquinone-induced Oxidative Damage in K562 Cells. Oxid Med Cell Longev. doi:10.1155/2016/3912515.

Jang, J. H. et al. (2016). APO-9′-fucoxanthinone extracted from undariopsis peteseniana protects oxidative stress-mediated apoptosis in cigarette smoke-exposed human airway epithelial cells. Mar Drugs. doi:10.3390/md14070140.

Ebeid, S. A. et al. (2016). Assessment of the radioprotective effect of propolis in breast cancer patients undergoing radiotherapy. New perspective for an old honey bee product. J Radiat Res Appl Sci. doi:10.1016/j.jrras.2016.06.001.

Wang, H, & Kim, N。 H。 (2016)。 CDK2 is required for the DNA damage response during porcine early embryonic development。 Biol Reprod。 doi:10。1095/biolreprod.116.140244.

Zhao, X. et al. (2016). Dioscin induces apoptosis in human cervical carcinoma HeLa and SiHa cells through ROS-mediated DNA damage and the mitochondrial signaling pathway. Molecules. doi:10.3390/molecules21060730.

Chen, X。 et al。 (2016)。 Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines。 Oncol Rep。 36:239-246。

Coleman, J。 et al。 (2016)。 Detecting Apoptosis, Autophagy, and Necrosis。 Apoptosis Methods in Toxicology 。 doi:10。1007/978-1-4939-3588-8_5。

Ding, Y. et al. (2016). Induction of ROS overload by alantolactone prompts oxidative DNA damage and apoptosis in colorectal cancer cells. Int J Mol Sci. doi:10.3390/ijms17040558.

Cui, F. M. et al. (2016). The role of miR-34a in tritiated water toxicity in human umbilical vein endothelial cells. Dose-Response. doi:10.1177/1559325816638585.

Laks, D. R. et al. (2016). Inhibition of nucleotide synthesis targets brain tumor stem cells in a subset of glioblastoma. Mol Cancer Ther. doi:10.1158/1535-7163.MCT-15-0982.

Abubakar, I. B. et al. (2016). Synergistic cytotoxic effects of combined δ-tocotrienol and jerantinine B on human brain and colon cancers. J Ethnopharmacol. doi:10.1016/j.jep.2016.03.004.

Hofstetter, C。 et al。 (2016)。 Inhibition of KDM6 activity during murine ESC differentiation induces DNA damage。J Cell Sci。 129:788-803。

Si, L。 et al。 (2016)。 Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion。 Eur J Pharmacol。 doi:10。1016/j。ejphar。2016。02。009。

Qu, L. et al. (2016). Corosolic acid analogue, a natural triterpenoid saponin, induces apoptosis on human hepatocarcinoma cells through mitochondrial pathway in vitro. Pharm Biol. doi:10.3109/13880209.2015.1104699.

Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. doi:10.1104/pp.15.00291.

Yuan, L。 et al。 (2015)。 Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells。 J Funct Foods。 20:496-505。

Ramy, N。 et al。 (2015)。 Jaundice, phototherapy and DNA damage in full-term neonates。  J Perinatol。  doi:10。1038/jp。2015。166。

Wu, C. F. et al. (2015). Anticancer activity of cryptotanshinone on acute lymphoblastic leukemia cells. Arch Toxicol. doi:10.1007/s00204-015-1616-4.

Kim, M. J. et al. (2015). Antibacterial effect and mechanism of high-intensity 405±5nm light emitting diode on Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus under refrigerated condition. J Photochem Photobiol B. 153:33-39.

Wang, X. et al. (2015). Enhancement of arabinocytosine (AraC) toxicity to AML cells by a differentiation agent combination. J Steroid Biochem Mol Biol.  doi:10.1016/j.jsbmb.2015.08.023.

Sun, X. et al. (2015). Electrochemical detection of 8-hydroxy-2′-deoxyguanosine as a biomarker for oxidative DNA damage in HEK293 cells exposed to 3-chloro-1, 2-propanediol. Anal Methods.doi:10.1039/C5AY01246E.

Neumann, J. et al. (2015). Mangrove dolabrane‐type of diterpenes tagalsins suppresses tumor growth via ROS‐mediated apoptosis and ATM/ATR–Chk1/Chk2‐regulated cell cycle arrest. Int J Cancer.  doi: 10.1002/ijc.29629.

Singh, A。 K。 et al。 (2015)。 Parental age affects somatic mutation rates in the progeny of flowering plants。 Plant Physiol。 168:247-257。

Hou, W. et al. (2015). The protecting effect of deoxyschisandrin and schisandrin B on HaCaT cells against UVB-induced damage. PLoS One. 10:e0127177.

Kim, M。 J。 et al。 (2015)。 Inactivation by 405±5 nm light emitting diode on Escherichia coli O157: H7, Salmonella Typhimurium, and Shigella sonnei under refrigerated condition might be due to the loss of membrane integrity。 Food Control。 doi:10。1016/j。foodcont。2015。05。012。

Haeger, S。 M。 et al。 (2015)。 Smad4 loss promotes lung cancer formation but increases sensitivity to DNA topoisomerase inhibitors。 Oncogene。  doi: 10。1038/onc。2015。112。

Ong, J。 Y。 et al。 (2015)。 2-Methoxy-1, 4-naphthoquinone (MNQ) induces apoptosis of A549 lung adenocarcinoma cells via oxidation-triggered JNK and p38 MAPK signaling pathways。 Life Sci。 doi: 10。1016/j。lfs。2015。03。019。

Prasad, M。 A。 et al。 (2015)。 Ebf1 heterozygosity results in increased DNA damage in pro-B cells and their synergistic transformation by Pax5 haploinsufficiency。 Blood。  doi: 10。1182/blood-2014-12-617282。

Obstoy, B. et al. (2015). Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study. BMC Pulm Med. 15:30.

Xiong, J。 et al。 (2015)。 Stemness factor Sall4 is required for DNA damage response in embryonic stem cells。J Cell Biol。 208:513-520。

Hu, Y. et al. (2015). Bile acids regulate nuclear receptor (nur77) expression and intracellular location to control proliferation and apoptosis. Mol Cancer Res. 13:291-292.

Gong, L. et al. (2015). p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Res. 25:351-369. 

Jin, L. et al. (2015). Association between oxidative DNA damage and the expression of 8-oxoguanine DNA glycosylase 1 in lung epithelial cells of neonatal rats exposed to hyperoxia. Mol Med Rep. doi: 10.3892/mmr.2015.3339.

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